Genomic DNA Extraction from Sperm

Document Type : Methodology Report


1 Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran


Unlike the somatic cells, sperm DNA is very compact due to replacement of histones with protamines. Disulfide bridges formed within and between the protamines inhibit the extraction of sperm DNA through standard techniques used for the somatic cells. Furthermore, the spermatozoa themselves are protected by a membrane which is rich in disulfide bonds, making cell lysis very difficult. Following a comprehensive literature search, we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion and PCR amplification.


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