TY - JOUR ID - 42449 TI - Genomic DNA Extraction from Sperm JO - Journal of Advanced Medical Sciences and Applied Technologies JA - JAMSAT LA - en SN - 2423-5903 AU - Anvar, Zahra AU - Namavar-Jahromi, Bahia AU - Ebrahimi, Samaneh AU - Gharesi-Fard, Behrouz AD - Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran Y1 - 2015 PY - 2015 VL - 1 IS - 2 SP - 120 EP - 121 KW - Sperm KW - Disulfide bonds KW - Chromatin KW - Histones KW - Protamines DO - 10.18869/nrip.jamsat.1.2.120 N2 - Unlike the somatic cells, sperm DNA is very compact due to replacement of histones with protamines. Disulfide bridges formed within and between the protamines inhibit the extraction of sperm DNA through standard techniques used for the somatic cells. Furthermore, the spermatozoa themselves are protected by a membrane which is rich in disulfide bonds, making cell lysis very difficult. Following a comprehensive literature search, we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion and PCR amplification. UR - https://jamsat.sums.ac.ir/article_42449.html L1 - https://jamsat.sums.ac.ir/article_42449_fa86225d79042e17a2a0801dc96fb3b0.pdf ER -