Pregnancy Outcome of Intracytoplasmic Sperm Injection in Relation to Duration of Cryopreservation of Spermatozoa Obtained through Testicular Sperm Extraction

Document Type : Original Articles

Authors

International Islamic Centre for Population Studies and Researches, Al Azhar University, Cairo, Egypt.

Abstract

Objectives: Freezing and in vitro culture of testicular spermatozoa or tissue are reliable approaches for the management of azoospermic patients and could all for the possibility of multiple IVF-ICSI procedures. However, the effect of cryopreservation duration on pregnancy outcome had been not fully examined. To analyze pregnancy outcome in patients underwent ICSI procedure in relation to duration of cryopreservation of the sperms. Materials: The present study included 62 couples who underwent to 255 cycles of ICSI; during the period from January 2013 to June 2014; in the International Islamic Centre for Population Studies and Researches. Couples of whom, male partner suffered from azoospermia underwent TESE for cryopreservation was included. Thawed testicular spermatozoa were used subsequently for an ICSI cycle. Women included in this study had also received routine infertility work-up. Frozen-thawed embryos were transferred on day 3–5. Clinical and ongoing pregnancy was defined as the presence of a gestational sac by transvaginal ultrasound in the 5th to 7th gestational week and the existence of a fetal heart beat at 12 weeks. Patient characteristics and pregnancy outcomes were documented.Results: The duration of cryopreservation had no effect on ICSI outcome, except significant difference between different groups as regard to cleavage rate (the higher cleavage rate was observed in second group, then fourth, fifth, first and third groups (it was 96.74±8.71, 94.50±8.82, 93.50±12.0, 92.35±12.47 and then 89.20±13.69 respectively). In addition, females in different groups were comparable as regard to personal characteristics.Conclusion: Duration of cryopreservation had no effect on pregnancy outcome.

Keywords

References

  1. Palermo G, Joris H, Devroey P, Van Steirteghem AC .Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992; 340(8810):17–8. doi: 10.1016/0140-6736(92)92425-f
  2. Bendikson KA, Neri QV, Takeuchi T, Toschi M, Schlegel PN, Rosenwaks Z, et al. The outcome of intracytoplasmic sperm injection using occasional spermatozoa in the ejaculate of men with spermatogenic failure. Journal of Urology. 2008; 180(3):1060–4. doi: 10.1016/j.juro.2008.05.025
  3. Corea M, Campagnone J, Sigman M. The diagnosis of azoospermia depends on the force of centrifugation. Fertility and Sterility. 2005; 83(4):920–2. doi: 10.1016/j.fertnstert.2004.09.028
  4. Nogueira D, Bourgain C, Verheyen G, Van Steirteghem AC. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies. Human Reproduction. 1999; 14(8):2041–9. doi: 10.1093/humrep/14.8.2041
  5. Wu B, Wong D, Lu S, Dickstein S, Silva M, Gelety TJ. Optimal use of fresh and frozen-thawed testicular sperm for intracytoplasmic sperm injection in azoospermic patients. Journal of Assisted Reproduction and Genetics. 2005; 22(11-12):389–94. doi: 10.1007/s10815-005-7481-y
  6. Park Y, Lee SH, Song SJ, Jun JH, Koong MK, Seo JT. Influence of motility on the outcome of in vitro fertilization/intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia. Fertility and Sterility. 2003; 80(3):526–30. doi: 10.1016/s0015-0282(03)00798-2
  7. Silber SJ, Nagy Z, Liu J, Tournaye H, Lissens W, Ferec C, et al. Genetics: The use of epididymal and testicular spermatozoa for intracytoplasmic sperm injection: The genetic implications for male infertility. Human Reproduction. 1995; 10(8):2031–43. doi: 10.1093/oxfordjournals.humrep.a136231
  8. Verheyen G, Nagy Z, Joris H, De Croo I, Tournaye H, Van Steirteghem A. Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes. International Journal of Gynecology & Obstetrics. 1997; 57(1):106–106. doi: 10.1016/s0020-7292(97)85625-1
  9. Gianaroli L, Magli1 MC, Selman HA, Colpi G ,Belgrano E, Trombetta C, et al. Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men. Human Reproduction. 1999; 14(4):1034–8. doi: 10.1093/humrep/14.4.1034
  10. Nicopoullos JDM, Gilling Smith C, Almeida PA, Ramsay JWA. The results of 154 ICSI cycles using surgically retrieved sperm from azoospermic men. Human Reproduction. 2004; 19(3):579–85. doi: 10.1093/humrep/deh092
  11. Giorgetti C, Chinchole J, Hans E, Charles O, Franquebalme J, Glowaczower E, et al. Crude cumulative delivery rate following ICSI using intentionally frozen–thawed testicular spermatozoa in 51 men with non-obstructive azoospermia. Reproductive BioMedicine Online. 2005; 11(3):319–24. doi: 10.1016/s1472-6483(10)60839-5
  12. Wald M, Ross LS, Prins GS, Cieslak-Janzen J, Wolf G, Niederberger CS. Analysis of outcomes of cryopreserved surgically retrieved sperm for IVF/ICSI. Journal of Andrology. 2006; 27(1):60–5. doi: 10.2164/jandrol.05076
  13. Huang C, Dong Q, Tiersch TR. Sperm cryopreservation of a live-bearing fish, the platyfish Xiphophorus couchianus. Theriogenology. 2004; 62(6):971–89. doi: 10.1016/j.theriogenology.2003.12.022
  14. Park YS, Kim MK, Lee SH, Cho JW, Song IO, Seo JT. Efficacy of testicular sperm chromatin condensation assay using aniline blue-eosin staining in the IVF-ET cycle. Clinical and Experimental Reproductive Medicine. 2011; 38(3):142. doi: 10.5653/cerm.2011.38.3.142
  15. Lee SH, Lee HS, Lim CK, Park YS, Yang KM, Park DW. Comparison of the clinical outcomes of day 4 and 5 embryo transfer cycles. Clinical and Experimental Reproductive Medicine. 2013; 40(3):122. doi: 10.5653/cerm.2013.40.3.122
  16. Silber SJ, Van Steirteghem A, Nagy Z. Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest. International Journal of Gynecology & Obstetrics. 1996; 55(3):317–317. doi: 10.1016/s0020-7292(97)82741-5
  17. Park YS, Kim MK, Lim CK, Lee SH, Park DW, Seo JT, et al. Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue. Journal of Assisted Reproduction and Genetics. 2014; 31(10):1331–6. doi: 10.1007/s10815-014-0304-2
  18. Sherman JK. Synopsis of the use of frozen human semen since 1964: state of the art of human semen banking. Fertility and Sterility. 1973; 24(5):397–412. doi: 10.1016/s0015-0282(16)39678-9
  19. Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: update on techniques, effect on dna integrity, and implications for ART. Advances in Urology. 2012; 2012:1–12. doi: 10.1155/2012/854837
  20. Bessonnat J, Brouillet S, Sintzel S, Gillois P, Bergues U, Boutte-Busquet C, et al. In cryptozoospermia or severe oligozoospermia is sperm freezing useful? Basic and Clinical Andrology. 2014; 24(1):15. doi: 10.1186/2051-4190-24-15
  21. Lahav-Baratz S, Rothschild E, Grach B, Koifman M, Shiloh H, Ishai D, Dirnfeld M. The value of sperm pooling and cryopreservation in patients with transient azoospermia or severe oligoasthenoteratozoospermia. Human Reproduction. 2002; 17(1):157–60. doi: 10.1093/humrep/17.1.157
  22. Oates RD, Lobel SM, Harris DH, Pang S, Burgess CM, Carson RS. Efficacy of intracytoplasmic sperm injection using intentionally cryopreserved epididymal spermatozoa. Human Reproduction. 1996; 11(1):133–8. doi: 10.1093/oxfordjournals.humrep.a019006
  23. Wood S, Thomas K, Schnauffer K, Troup S, Kingsland Ch, Lewis-Jones I .Reproductive potential of fresh and cryopreserved epididymal and testicular spermatozoa in consecutive intracytoplasmic sperm injection cycles in the same patients. Fertility and Sterility. 2002; 77(6):1162–6. doi: 10.1016/s0015-0282(02)03098-4
  24. Konc J, Kanyo K, Cseh S. Deliveries from embryos fertilized with spermatozoa obtained from cryopreserved testicular tissue. Journal of Assisted Reproduction and Genetics. 2006; 23(5):247–52. doi: 10.1007/s10815-006-9044-2
  25. Habermann H, Seo R, Cieslak J, Niederberger C, Prins GS, Ross L. In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa. Fertility and Sterility. 2000; 73(5):955–60. doi: 10.1016/s0015-0282(00)00416-7
  26. Lee SH, Song H, Park YS, Koong MK, Song IO, Jun JH. Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects on pregnancy outcomes. Fertility and Sterility. 2009; 91(3):798–804. doi: 10.1016/j.fertnstert.2007.12.061
  27. Tsai YR, Lan KC, Tsai CC, Lin PY, Kung FT, Liu YC, et al. Pregnancy outcome and neonatal data of children born after intracytoplasmic sperm injection with a different duration of cryopreservation of spermatozoa obtained through testicular sperm extraction. Taiwanese Journal of Obstetrics and Gynecology. 2013; 52(3):329–34. doi: 10.1016/j.tjog.2012.08.003
Journal of Advanced Medical Sciences and Applied Technologies
Volume 3, Issue 1 - Serial Number 1
11
March 2017
Pages 21-28

Files

Share

How to cite

Statistics