Journal of Advanced Medical Sciences and Applied Technologies

Journal of Advanced Medical Sciences and Applied Technologies

Assessment of the Neural-Like Cells Differentiation from Endometrial Stem Cells following Fluoxetine Treatment

Document Type : Original Articles

Authors
Morteza Motaghedifard
Hamid Reza Banafshe
Esmaeil Fakharian
Javad Verdi
Ali Reza Shoa-Hassani
Kashan, Mofateh street, Firestation street,Postal address: 8714649783
10.18869/nrip.jamsat.1.2.105
Abstract
Fluoxetine is a common antidepressant which selectively inhibits serotonin reuptake at synaptic level. Some research findings have proposed the effect this drug on neurogenesis, neuronal survival as well as proliferation of the neural progenitor cells. Endometrium is a part of uterus which harbors mesenchymal stem cells. This source of stem cells can be differentiated into neural cells which may potentially be used in treating many diseases. Given the above, this study was designed to assess the effect of fluoxetine on neural cells differentiation from endometrial stem cells. Endometrial stem cells obtained from stem cell banking were cultured in Dulbecco’s modified eagle’s medium (DMED) containing fetal bovine serum (FBS) in the presence of Retinoic Acid, fluoxetine and Retinoic Acid+fluoxetine for 10 days. To assess the differentiation of endometrial stem cells into neural-like cells, we used immunocytochemistry and RT-PCR. The viability of cells was assessed using the trypan blue test. Data analysis revealed that 61% of endometrial stem cells differentiated to neural-like cells. Moreover, the biopotency of neural-like cells on fluoxetine treatment was more pronounced across differentiation days. Based on our findings, fluoxetine was shown to be a suitable inducer for the differentiation of neural-like cells from endometrial stem cells.
Keywords
Endometrial stem cell
Neural-like cells
Fluoxetine
  1. Krabbe C, Zimmer J, Meyer M, Neural trans differentiation of mesenchymal stem cells a critical review. APMIS 2005; 113:831-44.
  2. Tysseling-Mattiace VM, Sahni V, Niece KL, Birch D, Czeisler C, Fehlings MG, Stupp SI, Kessler JA, Self-Assembling Nano fibers Inhibit Glial Scar Formation and Promote Axon Elongation after Spinal Cord Injury. The Journal of Neuroscience 2008; 28(14): 3814-23.
  3. Meng XE, Ichim T, Zhong J, Rogers A, Yin Z, Jackson J, Wang H, Ge W, Bogin V, Chan KW, Thebaud B, Riordan NH, Endometrial regenerative cells: A novel stem cell population. Journal of Translational Medicine 2007; 5:57-63.
  4. Gargett C, Masuda H: Adult stem cells in the endometrium. Molecular Human Reproduction 2012; 16 (11): 818-34.
  5. Gargett CE, Uterine stem cells: What is the evidence? Health Iran TRIAL ACCESS on December 25, 2011.
  6. Ai J, Tabatabaei FS, Jafarzadeh Kashi TS, Human Endometrial Adult Stem Cells May Differentiate Into Odontoblast Cells. Hypothesis 2009; 7(1): 21-9.
  7. Hida N, Nishiyama N, Miyoshi Sh, Kira Sh, Segawa k,Uyama T, Mori T, Miyado K, Ikegami Y, Cui Ch, Kiyono T, Kyo S, Shimizu T, Okano T, Sakamoto M, Ogawa S, Umezawa A, Novel Cardiac Precursor-Like Cells
  8. from Human Menstrual Blood-Derived Mesenchymal Cells. Stem cells 2008; 26:1695-04.
  9. Patel A, Park E, Kuzman M, Benetti F, Silva F, Allickson J, Multi potent Menstrual Blood Stromal Stem Cells: Isolation, Characterization, and Differentiation. Cell Transplantation 2008; 17: 303-11.
  10. Ai J, Tabatabaei FS, Kajbafzadeh AM, Myogenic Potential of Human Endometrial Adult Stem Cells. Iran J Med Hypotheses Ideas 2009; 3:25-32.
  11. Ai J, NorooziJavidan A, Mehrabani D, The Possibility of Differentiation of Human Endometrial Stem Cells into Neural Cells. IRCMJ 2010; 12(3):328-31.
  12. Wolff EF, Gao XB, Yao KV, Andrews ZB, Du H, Elsworth JD, Taylor HS, Endometrial stem cell transplantation restores dopamine production in a Parkinson’s diseasemodel. Journal of Cellular and Molecular Medicine 2011; 15(4): 74755.
  13. Shoae-Hassani A, Mortazavi-Tabatabaei SA, Sharif Sh, Rezaei-Khaligh H, Verdi J, DHEA provides a microenvironment for endometrial stem cells neurogenesis. Medical hypothesis 2011; 76:843-46.
  14. Duman RS, Nakagawa S, Malberg J, Regulation of Adult Neurogenesis by Anti depressant Treatment. Neuro psycho pharmacology. 2001; 25(6):836-44.
  15. Reif A, Fritzen S, Finger M, Strobel A, Lauer M, Schmitt A, Lesch KP, Neural stem cell proliferation is decreased in schizophrenia, but not in depression. Molecular Psychiatry 2006; 11: 514-22.
  16. TaupinP, Neurogenesis and the Effect of Antidepressants. Drug Target Insights 2006; 1: 13-7.
  17. Santarelli L, Saxe M, Gross C, Surget A, Battaglia F, Dulawa S , Requirement of hippocampal neurogenesis for the behavioral effects of antidepressants. Science 2003; 301: 805-9.
  18. Rahmani A, Kheradmand D, Keyhanvar P, Shoae-Hassani A, Darbandi-Azar A. Neurogenesis and Increase in Differentiated Neural Cell Survival via Phosphorylation of Akt1 after Fluoxetine Treatment of Stem Cells. BioMed Research International 2013; 2013: 9.
  19. Noureddini M., Verdi J., Mortazavi Tabatabaei S. A., Sharif S., Shoae Hassani A.. Human endometrial stem cell neurogenesis in response to NGF and bFGF. Cell Biology International, 2012; 36: 961–966.
  20. Pean JM, Menei P, Morel O, Montero-Menei CN, Benoit JP. Intra septalimplantation of NGF-releasing microspheres promotes the survival of axotomized cholinergic neuron. Biomaterials 2000; 21: 2097-01.
  21. Barber RP, Phelps PE, Houser CR. The morphology and distribution of neurons containing choline acetyltransferase in the adult rat spinal cord: an immunocytochemical study. J Comp Neural 1984; 229: 329-46.
  22. Bakhit C, Armanin M, Wong- Wai LT, Bennett GL, Wrathall JR, Increase in nerve growth factor-like immunoreactivity and decrease in choline acetyltransferase following contusive spinal cord injury. Brain Res 1991; 554: 264-71.
  23. Azizi SA, Stokes D, Augelli BJ, Digirolamo C, Prockop DJ. Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats-similarities to astrocyte grafts. Proc Natl Acad Sci USA 1998; 95(3): 3908-13.
  24. Lamoury FM, Croitoru-Lamoury J, Brew BJ. Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1. Cytotherapy 2006; 210(8): 228-42.
  25. Black IB, Woodburry D, Adult rat and human bone marrow stromal cells differentiate into neurons. Blood Cells Mol Dis 2001; 27(3): 632-36.
  26. Naghdi M, Tiraihi T, Mesbah-Namin SA, Arabkheradmand J. Transdifferentiation of bone marrow stromal cells into cholinergic neuronal phenotype: a potential source for cell therapy in spinal cord injury. Cythoterapy 2009; 11(2): 137-52.
Journal of Advanced Medical Sciences and Applied Technologies
Volume 1, Issue 2 - Serial Number 2
5
December 2015
Pages 105-111