Testicular Activity of Mice Treated With MeOH Extract of Achyranthes aspera Leaves

Abhaykumar Kamble, Chinnappa Reddy, Sharangouda Patil

Abstract


Objectives: In India control of fertility is based on the folk use of numerous Indian Traditional Plants that has been practiced for contraception from many years. Present investigation aims that, to evaluate the contraceptive potential of Achyranthes aspera leaves MeOH extract in male albino mice and has shown promising results of antispermatogenic, antiandrogenic and hypolipidaemic activities.
Materials & Methods: MeOH extract of A. aspera leaves at the doses of 25 mg and 50 mg/100 gm body weight was administered orally for 30 days to adult male albino mice. On day 31st, the rats were sacrificed and the testis were removed and weighed for gravimetrical analysis. Organs were processed for biochemical estimation, histological evaluation and histomorphometry for testicular analysis.
Results: Treatment with A. aspera leaves extract resulted in decrease the weight of testis 0.925±0.015 significantly (P<0.05). Morphometric analysis like diameter of testis 2554.22±18.545 seminiferous tubules 247.074±8.051 and Leydig cells 7.842±0.248 were decreased significantly (P<0.05) at 50mg/100gm body weight when compare to control. Spermatogenic elements like, spermatogonia, spermatocytes and spermatids in the testis were reduced significantly (P<0.05) as well as Leydig cells count in testis when compared to control. Biochemical analysis resulted as, protein, glycogen, and cholesterol content were decreased significantly (P<0.05) in testis of extracts treated mice, whereas alkaline phosphatase increased and acid phosphatase decreased when compared with that of control.
Conclusion: MeOH extract of A. aspera leaves arrest the spermatogenesis and inhibited testicular function in male mice without side effects to act as potential contraceptive.

 


Full Text:

PDF

References


Srivastav S, Singh P, Mishra G, Jha KK, Hosa RL. Achyranthes aspera-An important medicinal plant: A review.Journal of Natural Product and Plant Resources. 2011; 1:1-14.

Vijayan A, Liju VB, John JV, Reena, Parthipan B, Renuka C. Traditional remedies of Kani tribes of Kottoor reserve forest, Agasthyavanam, Thiruvananthapuram, Kerala. Indian Journal of Traditional Knowledge. 2007; 6(4):589-594.

Jain S, Choudhary GP, Jain DK. Medicinal plants with potential anti-fertility activity: A review. International Journal of Green Pharmacy. 2015; 9(4):223-228.

Meerwal P, Jain GC. Male fertility regulation with plant products: A review. International Journal of Pharmaceutical, Chemical and Biological Sciences. 2015; 5(1):146-162.

Devi P, Kumar P, Nidhi, Dhamija I. Antifertility activity of medicinal plants on male and female reproduction. International Journal of Pharmaceutical Sciences and Research. 2015; 6(3):988-1001.

National Institute of Science Communication and Information Resources. The wealth of India, raw materials: a ready reckoner on biodiversity and bioresources of India. New Delhi: National Institute; 1985.

Ratra PS, Misra KC. Seasonal variation in chemical composition of Achyranthes aspera Linn. and A. bidentata Bl. Indian Forester. 1970; 96(5):372-375.

Shibeshi W, Makonnen E, Debella A, Zerihun L. Phytochemical, contraceptive efficacy and safety Evaluations of the MeOH leaves extract of Achyranthes aspera L. in rats. Pharmacologyonline. 2006; 3:217-224.

Shibeshi W, Makonnen E, Zerihun L, Debella A. Effect of Achyranthes aspera L. on fetal abortion, uterine and pituitary weights, serum lipids and hormones. African Health Sciences. 2006; 6(2):108-112. doi: 10.5555/afhs.2006.6.2.108

Paul D, De D, Ali KM, Chattee K, Nandi DK, Ghosh D. Comparative study on the spermicidal activity of organic solvent fractions from hydroethanolic extracts of Achyranthes aspera and Stephania hernandifolia in human and rat sperm. Contraception. 2010; 81(4):355-361. doi: 10.1016/j.contraception.2009.09.001

Vasudeva N, Sharma SK. Post-coital antifertility activity of Achyranthes aspera Linn. root. Journal of Ethnopharmacology. Journal of Ethnopharmacology. 2006; 107(2):179–81. doi: 10.1016/j.jep.2006.03.009

Gurr E. Staining animal tissues, practical and theoretical. London: Leonard Hill; 1962.

Lowry OH, Rosenbrough NJ, Farr NL, Randall RJ. Protein measurement with folic phenol reagent. The Journal of Biological Chemistry. 1951; 193(1):265-75. PMID: 14907713

Carrol NV, Langelly RW, Row RH. Glycogen determination in liver and muscle by use of anthrone reagent. The Journal of Biological Chemistry. 1956; 220(2):583-93. PMID: 13331917

Peters JP, Van Slyke DD. Quantitative clinical chemistry. Philadelphia: Williams and Wilkins; 1946.

Bessey OA, Lowry OH, Brick NJ. A method for rapid determination of acid and alkaline phosphatase with 5 cu. mm. of serum. The Journal of Biological Chemistry. 1946; 164:321-329.

Deb C, Boral MC, Sarkar C. Measurement of hepatic parenchymal cell and nuclear volume in different classes of vertebrates. The Anatomical Record. 1964; 148(3):499–501. doi: 10.1002/ar.1091480307

Abercrombie M. Estimation of nuclear population from microtome sections. The Anatomical Record. 1946; 94(2):239–47. doi: 10.1002/ar.1090940210

SPSS Inc. SPSS for Windows, Rel. 11.0.1. 2001. Chicago: SPSS Inc.; 2001.

De Cássia da Silveira e Sá R, Leite MN, de Moura Reporedo M, Nóbrega de Almeida R. Evaluation of long-term exposure to Mikania glomerata (Sprengel) extract on male Wistar rats’ reproductive organs, sperm production and testosterone level. Contraception. 2003; 67(4):327–31. doi: 10.1016/s0010-7824(02)00523-1

Mishra RK, Singh SK. Antispermatogenic and antifertility effects of fruits of Piper nigrum L. in mice. Indian Journal of Experimental Biology. 2009; 47(9):706-14. PMID: 19957882

Ghosh S, Bartke A, Grasso P, Reichert LE, Russell LD. Structural response of the hamster Sertoli cell to hypophysectomy: A correlative morphometric and endocrine study. The Anatomical Record. 1992; 234(4):513–29. doi: 10.1002/ar.1092340407

Vijaykumar B, Sangamma I, Sharanabasappa A, Patil SB. Antifertility activity of various extracts of Crotalaria juncea Linn. seeds in male mice. Philippine Journal of Science. 2003; 132(1):39-46.

Rington JH, Armstrong DT. Follicle-stimulating hormone stimulates estradiol-17beta synthesis in cultured Sertoli cells. Proceedings of the National Academy of Sciences. 1975; 72(7):2677–81. doi: 10.1073/pnas.72.7.2677

Seetharam YN, Sujeeth H, Jyothishwaran G, Barad A, Sharanabasappa G, Umareddy B, et al. Antifertility effect of ethanolic extract of Amalakyadi churna in male albino mice. Asian Journal of Andrology. 2003; 5(3):247-50. PMID: 12937810

Rato L, Alves MG, Socorro S, Duarte AI, Cavaco JE, Oliveira PF. Metabolic regulation is important for spermatogenesis. Nature Reviews Urology. 2012; 9(6):330–8. doi: 10.1038/nrurol.2012.77

Prabu SM, Sumedha N. Diallyl trisulfide (DATS) abrogates arsenic induced testicular oxidative stress in rats. International Journal of Pharmacology and Toxicology. 2014; 2(2):30-37. doi: 10.14419/ijpt.v2i2.2517

Lii CK, Huang CY, Chen HW, Chow MY, Lin YR, Huang CS, et al. Diallyl trisulfide suppresses the adipogenesis of 3T3-L1 preadipocytes through ERK activation. Food and Chemical Toxicology. 2012; 50(3-4):478–84. doi: 10.1016/j.fct.2011.11.020

Malashetty VB, Sharanbasappa A, Patil SB. Spermatogenic index and hormonal profile in the rats received chromatographic fractions of ethanol extract of Crotalaria juncea L. seeds. Oriental Pharmacy and Experimental Medicine. 2006; 6: 86-95.

Reton S, Smith PJS, Lui B, Brown D. Acidification of the male reproductive tract by a proton pumping(H+)-ATPase. Nature Medicine. 1996; 2(4):470–2. doi: 10.1038/nm0496-470

Nair N, Edwards MS, Bedwall RS, Mathur RS. Effect of adrenalectomy and adrenalectomy-hydrocortisone treatment on zinc, biochemical parameters and histology of testes of rats. Indian Journal of Experimental Biology. 1987; 25(10):651-9. PMID: 3450587

Raji Y, Bolarinwa AF. Antifertility activity of Quassia amara in male rats in vivo study. Life Sciences. 1997; 61(11):1067–74. doi: 10.1016/s0024-3205(97)00615-2

Aleem M, Choudhari J, Padwal V, Balasinor N, Parte P, Gill-Sharma MK. Hyperprolactinemia affects spermiogenesis in adult male rats. Journal of Endocrinological Investigation. 2005; 28(3):39–48. doi: 10.1007/bf03345528

Fransworth NR, Wallerm DP. Current status of plant products reported to inhibit sperm. Research Frontiers in Fertility Regulation. 1982; 2(1):1-16. PMID: 12179631

Sarkar SN, Majumdar AC, Chattopadhyay SK. Effect of isoproturon on male reproductive system: clinical, histological and histoenzymological studies in rats. Indian Journal of Experimental Biology. 1997; 35(2):133-8. PMID: 9315220

Gupta RS, Kumar P, Dixit VP, Dobhal MP. Antifertility studies of the root extract of the Barleria prionitis Linn in male albino rats with special reference to testicular cell population dynamics. Journal of Ethnopharmacology. 2000; 70(2):111–7. doi: 10.1016/s0378-8741(99)00150-6

Reddy CM, Murthy DRK, Patil SB. Antispermatogenic and androgenic activities of various extracts of Hibiscus rosa sinensis in albino mice. Indian Journal of Experimental Biology. 1997; 35(11):1170-4. PMID: 9567744

Russell LD, Alger LE, Nequin LG. Hormonal control of pubertal spermatogenesis. Endocrinology. 1987; 120(4):1615–32. doi: 10.1210/endo-120-4-1615

Haneji T, Maekawa M, Nishimune Y. Vitamin A and FSH synergistically induce differentiation of type A spermatogonia in adult mouse cryptorchid testes in vitro. Endocrinology. 1984; 114(3):801–5. doi: 10.1210/endo-114-3-801

Hall PF. Testicular steroid synthesis: Organization and regulation. In: Knobil E, Neill JD, editors. Physiology of Reproduction. New York: Raven Press; 1994.




DOI: http://dx.doi.org/10.18869%2Fnrip.jamsat.3.2.93

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

pISSN: 2423-5903                                             eISSN: 2538-4473