Comparing the Effects of Human and Fetal Bovine Serum on Mesenchymal Stem Cells Under Oxidative Stress

Vahid Razban, Maryam Hosseinipour, Mehdi Mahmoodi, Mohammad Reza Hajizadeh, Hossein Mohammadpour, Giti Farsi, Ali Reza Khoshdel

Abstract


Objectives: Stem cells are undifferentiated cells capable of creating different types of cell in the body. Stem cell proliferation often is performed in the culture medium supplemented with Fetal Bovine Serum (FBS). Unknown compounds in the FBS, risk of contamination and disease transmission encourages the researches toward finding an alternative to FBS. Several factors are involved in the Mesenchymal Stem Cells (MSCs) precocious death in the transplanted tissue environment. Oxidative Stress (OS) is one of the main causes of stem cell apoptosis in the initial days after transplantation. The aim of this study was to evaluate the effect of Human Serum (HS) on the viability and oxidative related enzymes in human Adipose tissue-Derived Stem Cells (ADSCs) under oxidative stress in comparison with FBS.
Materials & Methods: Human serum were obtained from blood of a healthy donor persons, in respective intervals during few days. The ADSCs were isolated from lipolysis operation samples and their cuture media were supplemented with FBS or HS and different concentrations of H2O2 as the oxidative agent.
Results: The results showed that cell proliferation and viability of ADSCs under oxidative stress condition was significantly higher in the culture medium supplemented with HS in comparison with FBS supplemented medium (P<0.05).
Conclusion: This study showed that FBS could be replaced by HS in MSC culture medium with improved effects on cell proliferation and oxidative related enzyme activity under oxidative Stress condition.


Full Text:

PDF

References


Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells: Characterization, differentiation, and application in cell and gene therapy. Journal of Cellular and Molecular Medicine. 2004; 8(3):301–16. doi: 10.1111/j.1582-4934.2004.tb00320.x

Razban V, Lotfi AS, Soleimani M, Ahmadi H, Massumi M, Khajeh S, et al. HIF-1α overexpression induces angiogenesis in mesenchymal stem cells. BioResearch Open Access. 2012; 1(4):174–83. doi: 10.1089/biores.2012.9905

Squillaro T, Peluso G, Galderisi U. Clinical trials with mesenchymal stem cells: An update. Cell Transplantation. 2016; 25(5):829–48. doi: 10.3727/096368915x689622

Razban V, Khajeh S, Lotfi AS, Mohsenifar A, Soleimani M, Khoshdel A, et al. Engineered heparan sulfate-collagen IV surfaces improve human mesenchymal stem cells differentiation to functional hepatocyte-like cells. Journal of Biomaterials and Tissue Engineering. 2014; 4(10):811–22. doi: 10.1166/jbt.2014.1234

Sahebghadam Lotfi A. High yield generation of hepatocyte like cells from adipose derived stem cells. Scientific Research and Essays. 2012; 7(10):1141-7. doi: 10.5897/sre11.1437

Keating A. Mesenchymal stromal cells. Current Opinion in Hematology. 2006; 13(6):419–25. doi: 10.1097/01.moh.0000245697.54887.6f

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F., Krause DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315–7. doi: 10.1080/14653240600855905

Khairoun M, S Korevaar S. Human bone marrow- and adipose tissue-derived mesenchymal stromal cells are immunosuppressive in vitro and in a humanized allograft rejection model. Journal of Stem Cell Research & Therapy. 2013; doi: 10.4172/2157-7633.s6-001

Denu RA, Hematti P. Effects of oxidative stress on mesenchymal stem cell biology. Oxidative Medicine and Cellular Longevity. 2016; 2016:1–9. doi: 10.1155/2016/2989076

Gstraunthaler G. Alternatives to the use of fetal bovine serum: Serum-free cell culture. Altex. 2003; 20(4):275-81. PMID: 14671707

Fernandes-Platzgummer A, Carmelo JG, da Silva CL, Cabral JMS. Clinical-grade manufacturing of therapeutic human mesenchymal stem/stromal cells in microcarrier-based culture systems. Methods in Molecular Biology. 2016; 375–88. doi: 10.1007/978-1-4939-3584-0_22

Bieback K, Hecker A, Kocaömer A, Lannert H, Schallmoser K, Strunk D, et al. Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells. 2009; 27(9):2331–41. doi: 10.1002/stem.139

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: The serum-free media interactive online database. Altex. 2010; 27(1):53-62. PMID: 20390239

Van der Valk J, Brunner D, De Smet K, Fex Svenningsen Å, Honegger P, Knudsen LE, et al. Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods. Toxicology in Vitro. 2010; 24(4):1053–63. doi: 10.1016/j.tiv.2010.03.016

Taupin P. Derivation of embryonic stem cells for cellular therapy: Challenges and new strategies. Medical Science Monitor. 2006; 12(4):75-8.

Kobayashi T. Motility and growth of human bone-marrow mesenchymal stem cells during ex vivo expansion in autologous serum. Journal of Bone and Joint Surgery - British Volume. 2005; 87(10):1426–33. doi: 10.1302/0301-620x.87b10.16160

Wang Z, Zhang F, Wang L, Yao Y, Zhao Q, Gao X. Lipopolysaccharides can protect mesenchymal stem cells (MSCs) from oxidative stress-induced apoptosis and enhance proliferation of MSCs via toll-like receptor (TLR)-4 and PI3K/Akt. Cell Biology International. 2009; 33(6):665–74. doi: 10.1016/j.cellbi.2009.03.006

Wang X, Zhao T, Huang W, Wang T, Qian J, Xu M, et al. Hsp20-engineered mesenchymal stem cells are resistant to oxidative stress via enhanced activation of Akt and increased secretion of growth factors. Stem Cells. 2009; 27(12):3021-31. doi: 10.1002/stem.230.

Findeisen HM, Pearson KJ, Gizard F, Zhao Y, Qing H, Jones KL, et al. Oxidative stress accumulates in adipose tissue during aging and inhibits adipogenesis. PLoS ONE. 2011; 6(4):18532. doi: 10.1371/journal.pone.0018532

Altaner C, Altanerova V, Cihova M, Ondicova K, Rychly B, Baciak L, et al. Complete regression of glioblastoma by mesenchymal stem cells mediated prodrug gene therapy simulating clinical therapeutic scenario. International Journal of Cancer. 2013; 134(6):1458–65. doi: 10.1002/ijc.28455

Cucchiarini M, Venkatesan JK, Ekici M, Schmitt G, Madry H. Human mesenchymal stem cells overexpressing therapeutic genes: From basic science to clinical applications for articular cartilage repair. Bio-Medical Materials and Engineering. 2012; 22(4):197-208.

Keyßer G, Müller L, Schendel M, Schmoll H-J. [Therapeutic use of mesenchymal stromal cells in autoimmune diseases (German)]. Zeitschrift für Rheumatologie. 2009; 68(3):220–7. doi: 10.1007/s00393-008-0394-2

Seo JH, Cho S-R. Neurorestoration induced by mesenchymal stem cells: Potential therapeutic mechanisms for clinical trials. Yonsei Medical Journal. 2012; 53(6):1059. doi: 10.3349/ymj.2012.53.6.1059

Valle-Prieto A, Conget PA. Human mesenchymal stem cells efficiently manage oxidative stress. Stem Cells and Development. 2010; 19(12):1885–93. doi: 10.1089/scd.2010.0093


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.