ORIGINAL_ARTICLE
A Survey on the Role of Fetal Microchimerism in the Maternal Body
Microchimerism is explained as the simultaneous presence of a few foreign cells with different genetic origins of different individuals in a person. Transfer of these cells through blood transfusion, organ transplantation and particularly the mutual transfer of cells between the mother and fetus during pregnancy is possible. This article is an overview of the role of fetal cell microchimerism in maternal health and disease, especially autoimmune disorders and cancer. The original and related articles were found by search in PubMed, Scopus, Springer, Sciencedirect with an emphasis on literature published in the period 2000 to 2015. It was concluded that microchimeric cell can play different roles in maternal body, including natural microchimerism (bearing no biological role), utility (damaged tissue repair), and pathogenesis (causing autoimmune disease and cancer). Further studies and more in-depth knowledge about these cells may help explaining their new roles and using them in treatment or determining the prognosis of various diseases.
https://jamsat.sums.ac.ir/article_42446_bc2016553908fa08cd31bd528e92657b.pdf
2015-12-30
68
77
10.18869/nrip.jamsat.1.2.68
Fetal microchimerism
Autoimmune Diseases
cancer
Farzaneh
Bozorg-Ghalati
fbozorgghalati@yahoo.com
1
PhD student of molecular medicine, school of advanced technologies in medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
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102
ORIGINAL_ARTICLE
DFT and Molecular Docking Studies on Biological Active Acridines: the Interaction with Bovine Serum Albumin
Acridine derivatives, especially 1,8-dioxo-9-aryl-decahydroacridine represent significant scaffolds in medicinal chemistry. Given the biological properties of such products which are used in drug development, they need to have appropriate carrier. Proteins are generally used as helpful tools in drug delivery. Consequently, molecular docking between these compounds and bovine serum albumin (BSA) has been taken into account. Furthermore, in order to achieve better results, the suggested compounds have been optimized using Gaussian 03 software.
https://jamsat.sums.ac.ir/article_42450_8c43af31e244df42c43ddf9a48bbafa5.pdf
2015-12-30
78
85
10.18869/nrip.jamsat.1.2.78
DFT study
molecular docking
Biological active
Anti-tumor properties
BSA
Ali
Amani
ali_amani1383@yahoo.com
1
a) Department of medical nanotechnology, school of advanced medical sciences and technologies, Shiraz University of Medical Sciences, Shiraz, Iran
b) Department of chemistry Shiraz University of technology Shiraz Iran
C)Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
Saeed
Gholamzadeh
2
AUTHOR
Mohammad
Zarenezhad
3
AUTHOR
Abdorrasoul
Malekpour
4
AUTHOR
Ehsan
Javidnezhad
5
AUTHOR
Sharma O, Singla RK, Shrivastava B. Scientific Assessment of Antioxidant potential of Pyrimidine-2, 4 (1H, 3H)-diones. Indo Glob J Pharm Sci. 2012;2(2):142-6.
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Antonini I, Polucci P, Magnano A, Martelli S. Synthesis, Antitumor Cytotoxicity, and DNA-Binding of Novel N-5, 2-Di (Ï-aminoalkyl)-2, 6-dihydropyrazolo [3, 4, 5-kl] acridine-5-carboxamides. J Med Chem. 2001;44(20):3329-33.
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Morris GM, Lim-Wilby M. Molecular docking. Molecular Modeling of Proteins: Springer; 2008. p. 365-82.
9
Anbazhagan R, Sankaran K. Syntheses, spectral characterization, single crystal X-ray diffraction and DFT computational studies of novel thiazole derivatives. J Mol Struct. 2013;1050:73-80.
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Ä°nkaya E, Dinçer M, Ekici Ã, Cukurovali A. Nâ²-(2-methoxy-benzylidene)-N-[4-(3-methyl-3-phenyl-cyclobutyl)-thiazol-2-yl]-chloro-acetic hydrazide: X-ray structure, spectroscopic characterization and DFT studies. J Mol Struct. 2012;1026:117-26.
11
Güntepe F, SaraçoÄlu H, ÃalıÅkan N, Yüksektepe Ã, Cukurovali A. Synthesis, molecular and crystal structure analysis of 2-bromo-4-chloro-6-{[4-(3-methyl-3-phenyl-cyclobutyl)-thiazol-2-yl]-hydrazonomethyl}-phenol by experimental methods and theoretical calculations. J MolStruct. 2012;1022:204-10.
12
Teng Y, Liu R, Li C, Xia Q, Zhang P. The interaction between 4-aminoantipyrine and bovine serum albumin: multiple spectroscopic and molecular docking investigations. J. Hazard. Mater. 2011;190(1):574-81.
13
Shahabadi N, Hadidi S. Molecular modeling and spectroscopic studies on the interaction of the chiral drug venlafaxine hydrochloride with bovine serum albumin. Spectrochim. Acta. A. 2014;122:100-6.
14
Kumari M, Maurya JK, Singh UK, Khan AB, Ali M, Singh P, et al. Spectroscopic and docking studies on the interaction between pyrrolidinium based ionic liquid and bovine serum albumin. Spectrochim. Acta. A. 2014;124:349-56.
15
ORIGINAL_ARTICLE
Neuronal Spines Can be Affected by Static Magnetic Fields: The Impact on Microtubule Dynamic Nature
Recently, the hypothesis in which memory and information would be stored as magnetic forms in astrocytes is expanding and neuromagnetic interactions between neurons and neighboring astrocytes in neocortex have potential to be the basis of memory formation. It has been proposed that all sorts of information may be maintained in form of neuronal activity-associated magnetic fields (NAAMFs) and thereby alterations of magnetic fields in the brain may potentially affect the memory function. On the other hand, microtubules (MTs), the most essential elements of cytoskeleton, are crucial in regulation of spine development and morphology, brain cognitive behavior, consciousness and information storage. Because of MT dynamic nature, it can produce local magnetic field in neurons through vibration. According to size, number, structure and function of microtubule proteins, they are the most eligible components of neurons to be affected by endogenous and exogenous magnetic fields. In this study we tried to investigate the possible effects of exogenous static magnetic fields (SMFs) on memory through examining the structural and functional changes in MT dynamic activity and neural cell morphology. MT activity results revealed that MT polymerization process was not attained to steady state at the right time in the presence of SMF at 300 mT and the ascending slope at the steady state phase was found as abnornmal. In addition, MT structure was relatively changed. On the influence of SMF, PC12 neuron-liked cells’ spines decreased significantly and their morphology altered to pyramidal form.
https://jamsat.sums.ac.ir/article_42451_58687b4542a8c58976a27d63f3db6344.pdf
2015-12-31
86
92
10.18869/nrip.jamsat.1.2.86
Microtubule polymerization
Tubulin structure
Static Magnetic Fields
PC12 cells
Neocortical magnetic communication
Ali
Dadras
a_dadras@behestandarou.com
1
IBB, Tehran University
LEAD_AUTHOR
Deyhim
Atarod
a_dadras@behestanddarou.com
2
IBB, Tehran University
AUTHOR
Ali
Afrasiabi
3
IBB, Tehran University
AUTHOR
Atiye
Liaghi
4
IBB, Tehran University
AUTHOR
Ali
Naghshineh
5
IBB, Tehran University
AUTHOR
Gholam Hossein
Riazi
6
IBB, Tehran University
AUTHOR
Verkhratsky A. Physiology of neuronal-glial networking. Neurochem Int. 2010 Nov;57(4):332-43.
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55
ORIGINAL_ARTICLE
A Comparison of Stress Reactions, Coping Styles, Subjective Well-Being and Its Sub-Scales in Fertile and Infertile Women
Background: Infertility is described nearly universally as an extremely stressful condition with enduring effects on the marriage as well as each partner’s well-being. The present study was done to compare stress reactions, coping styles, subjective well-being and its sub-scales in fertile and infertile women. Materials and Methods: This analytic cross-sectional study enrolled 120 women (60 fertile and 60 infertile). The stress symptoms questionnaire, coping inventory of stressful situations-short form and subjective well-being questionnaire were administered as assessment tools. Results: Our results demonstrated significant difference among infertile and fertile women in stress reactions, coping strategies and subjective well-being. Infertile women reacted more than fertile women emotionally, cognitively and behaviorally. Infertile women used more emotion-oriented coping methods. Infertile and normal subjects differed significantly in terms of emotional, psychological and social well-being. Conclusion: Given the psychological consequences of infertility which may exaggerate the course and cause delayed treatment responses, psychological interventions seem to be clinically warranted.
https://jamsat.sums.ac.ir/article_42452_6b1e03a45766a479632d9c52889643d8.pdf
2015-12-31
93
97
10.18869/nrip.jamsat.1.2.93
Stress reactions
Coping styles
Subjective well-being
Fertility
infertility
Vajiheh
Sadat Tovliat
1
AUTHOR
Mahshad
Motaghedifard
mahshad_mf@yahoo.com
2
AUTHOR
Mohammad Reza
Tamannaeifar
tamannai@kashanu.ac.ir
3
LEAD_AUTHOR
Fassino S, Piero A, Boggio B, Piccioni V, and Garzaro L. Anxiety, depression and anger suppression in infertile couples: a controlled study. Human Reproduction 2002; 11: 2986â2994.
1
Kemeter P. Studies on psychosomatic implications of infertilityâ effects of emotional stress on fertilization and implantation in in-vitro fertilization. Hum Reprod 1988; 3: 341â352.
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Gallinelli AR, Roncaglia ML, Matteo I, Ciaccio A. Volpe, and Facchinetti F. Immunological changes and stress are associated with different implantation rates in patients undergoing in vitro fertilizationâ embryo transfer. Fertil Steril 2001; 76: 85â91.
3
Demyttenaere KP, Nijs G, Evers-Kiebooms, and Koninckx PR. Coping and the ineffectiveness of coping influence the outcome of in vitro fertilization through stress responses. Psychoneuroendocrinology 1992; 17: 655â665.
4
Hendrick V, Gitlin M, Altshuler L, and Korenman S. Antidepressant medications, mood and male fertility. Psychoneuroendocrinology 2000; 25: 37â51.
5
Klonoff-Cohen HE, Chu L, Natarajan, and Sieber W. A prospective study of stress among women undergoing in vitro fertilization or gamete intrafallopian transfer. Fertil Steril 2001; 76: 675â687.
6
Davis D, and Deraman CN. Coping strategies of infertile women. J ObsGyn Neonatal Nurs, 1991; 20(3): 221-8.
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Williams ME. Toward greater understanding of the psychological effects of infertility on women. Psychotherapy in Private Practice 1997; 16: 3, 7â26.
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Pottinger CJ, McKenzie V, Fredericks S, Wynter D, Everett, and Walters Y. Gender Differences in Coping with Infertility among Couples Undergoing Counselling for In Vitro Fertilization Treatment. West Indian Med J 2006; 4: 237-242.
9
Rashidi BS, Hoseini P, Beigi M, Ghazizadeh, and Farahani M. Infertility Stress: The Role of Coping Strategies, Personality Trait, and Social Support. Journal of Family and Reproductive Health 2011; 4: 101-108.
10
Shokri A. Comparison on of causal relationship of female and male studentsâ academic stress and consequences in individualistic and collectivist cultural paradigms. 2010 Doctoral Dissertation. Tarbiat Moalem University, Tehran.
11
Tao PR, Coates, and Maycock B. Investigating marital relationship in infertility: a systematic review of quantitative studies. J Reprod Infertil 2012; 2:71-80.
12
Lansakara NA, Wickramasinghe R, and Seneviratne HR. Feeling the blues of infertility in a South Asian context: psychological well-being and associated factors among Sri Lankan women with primary infertility. Women Health 2011; 4: 383-99.
13
Tamannaeifar, M. Motaghedifard M. Subjective well-being and its sub-scales among students: The study of role of creativity and self-efficacy. Thinking Skills and Creativity 2014; 12: 37â 42.
14
World Health Organization. Current Practices and Controver-sies in Assisted Reproduction. Report of a meeting on Medical, Ethical and Social Aspects of Assisted Reproduction. Geneva, Switzerland: 2002: 15â396.
15
Faal Kalkhoran LH, Bahrami NA, Farrokhi H, Zeraati, and Tarahomi M. Comparing Anxiety, Depression and Sexual Life Satisfaction in Two Groups of Fertile and Infertile Women in Tehran. J Reprod Infertil 2011; 2:157-163.
16
Bak CW, Seok HH, Song S, Kim ES, Sun HY, & Yoon TK. Hormonal Imbalances and Psychological Scars Left Behind in Infertile Men. Journal of Andrology 2012; 2: 181-189.
17
Abbasi Molid H, Rezazadeh Varaghchi J. Psychological meta-analysis of the infertility researches in Iran. IJOGI, 2012; 13: 26-33.
18
Dhillon RC, Cumming E, and Cumming DC. Psychological well-being and coping patterns in infertile men. Fertili Steril 2000; 4: 702-6.
19
Inhorn MC. Sexuality, masculinity and infertility in the Egypt: potent troubles in the marital and medical encounter. J Mens Stud 2002; 3: 343- 59.
20
Hassanpoor B, Simbar M, Vadahyre A, Hossein rashidy B. Explanation psychology impacts of infertility in seeking treatment infertile women, Qualitative study. Journal Research College of nursing and midwifery of shayid Beheshti University 2014; 83: 1-8.
21
Gibson DM, and Myers JE. Gender and Infertility: a relational approach to counseling women. J counseling 2000; 4: 11-40.
22
KormiNouri R. Psycho-social aspects of infertility. J ReprodInfertil 2000; 2:57-68.
23
Van den Akker OB. Coping, quality of life and psychological symptoms in three groups of sub-fertile women. Patient Educ Couns 2005; 2:183-9.
24
Faramarzi MH, Pasha S, Esmaelzadeh G, jorsarai M, Aghajani Mir Sh, Abedi. Is coping strategies predictor of anxiety and depression in couple infertile? Scientific Research Corporation 2013; 3A: 643-649.
25
Aflakseir A, and Zarei M. Association between Coping Strategies and Infertility Stress among a Group of Women with Fertility Problem in Shiraz, Iran. Journal of Reproduction & Infertility 2013; 4:202-206
26
Bakhshayesh AR, Kazeraninejad M, Dehghan Mongabadi M, Raghebian M. A comparison of general health and coping strategies in fertile and infertile women in Yazd. Iranian Journal of Reproductive Medicine 2012; 6: 601-606.
27
Joshi HL, Singh, and Bindu R. Psychological Distress, Coping and Subjective Wellbeing among Infertile Women. Journal of the Indian Academy of Applied Psychology 2009; 2: 329-336.
28
Cousineau TM, and Domar AD. Psychological impact of infertility. Best Practice & Research Clinical Obstetrics & Gynaecology 2007; 2:293- 308.
29
Sami N, and Tazeen SA. Perceptions and Experiences of Women in Karachi- Pakistan Regarding Secondary Infertility: Results from a Community-Based Qualitative Study. Bstetrics and Gynecology International < http://www.hindawi.com/journals/ogi/2012/108756>. [4 Nov 2012].
30
Hammerli KZ, Hansjorg J, Barth. The efficacy of psychological interventions for infertile patients: A meta-analysis examining mental health and pregnancy rate. Human Reprod Update 2009; 3: 279-295.
31
Dyer SJ. Psychological and social aspects of infertility in developing countries. International Journal of Gynecology & Obstetrics 2009; 2: 25-26.
32
Sudha GK, Reddy SN, Reddy KN, Reddy BKC. Emotional distress in infertile couples: Across-cultural study. Asia-Pacific journal Social Science 2011; 1:90-101.
33
ORIGINAL_ARTICLE
Nano-TiCl4.SiO2 Promoted One-Pot Synthesis of Amides or Nitriles from Aldehydes
This research proposes an efficient and easy procedure for the preparation of amides or nitriles from aldehydes. Such materials were prepared via one-pot three component condensation reaction of various aldehydes in the presence of nano-TiCl4.SiO2 as a catalyst under thermal conditions. All reactions proceeded in high yields and in a moderately short reaction time. Meanwhile, oximes were obtained from aldehyeds in the presence of nano-TiCl4.SiO2.
https://jamsat.sums.ac.ir/article_42453_035eba2df3fcc39285a9b5e5f1b720c9.pdf
2015-12-31
98
104
10.18869/nrip.jamsat.1.2.98
Nitrile
Oxime
Amid
Nano-ToCl4.SiO2
Leila
Zamani
l.zamani2008@gmail.com
1
LEAD_AUTHOR
B. F.
Mirjalili
2
Department of Chemistry, College of Science, Yazd University, Yazd,PO Box 89195-741, I. R. Iran
AUTHOR
Humphrey JM, Chamberlin AR. Chemical Synthesis of Natural Product Peptides: Coupling Methods for the Incorporation of Noncoded Amino Acids into Peptides. Chemical Reviews., 1997,. 97(6): 2243- 2266.
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Beckmann E. Zur Kenntniss der Isonitrosoverbindungen. Deutsche Chemische Gesellschaft., 1886,. 19(1): 988-993.
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Fabiani ME. Crystal and Molecular Structure of 4-Benzoyl-1,5-diphenyl-1H-pyrazole-3-carbonitrile. Drug News Perspect., 1999,. 12(4): 207-216.
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Chichiro M, Nagamoto H, Takemura I, Kitano K, Komatsu H, Sekiguchi K, Tabusa F, Mori T, Tominnaga M, Yabuuchi Y. Solvent-free Synthesis of Nitriles from Aldehydes Catalyzed by KF/Al2O3, Montmorillonite KSF and K10 J. Med. Chem., 1995,. 38(2): 353-358.
4
Diana G, Catcliffe D, Volkots DL, Mallamo JP, Bailey TR, Vescio N, Oglesby RC, Nitz TJ, Wetzel J, Giranda V, Pevear DC, Dutko F.J. Antipicornavirus activity of tetrazole analogs related to disoxaril. J. Med. Chem., 1993,. 36(22): 3240-3250.
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Martinez-Asencio A, Yus M, Ramon DJ. Copper(II) acetate-catalyzed one-pot conversion. Tetrahedron., 2012,. 68(21): 3948-3951.
6
Sridhar M, Narsaiah C, Sairam VV, Reddy G.K, Raveendra J, Reddy MKK, Ramanaiah BC. Microwave-assisted efficient one-step synthesis of amides from ketones and benzoxazoles from (2-hydroxyaryl) ketones with acetohydroxamic acid using sulfuric acid as the catalyst. Tetrahedron Lett., 2011,. 52(46): 6103-6107.
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Liang J, Lv J, Shan ZC. Metal-free synthesis of amides by oxidative amidation of aldehydes with amines in PEG/oxidant system. Tetrahedron., 2011,. 67(44): 8532-8535.
8
Movassagh B, Shokri S. An efficient and convenient KF/Al2O3 mediated synthesis of nitriles from aldehydes. Tetrahedron Lett., 2005,. 46(40): 6923-6925.
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Telvekar VN, Patel KN, Kundaikar HS, Chaudhari HK. An efficient one-pot protocol for the conversion of benzaldehydes into tetrazole analogs. Tetrahedron Lett., 2008,. 49(14): 2213-2215.
10
Zamani L, Mirjalili BF, Zomorodian K, Namazian M, Khabnadideh S, Faghih mirzaei E. SiO2 AS ANTIFUNGAL AGENTS AND TAUTOMERISM THEORETICAL STUDY OF SOME PRODUCTS. FARMACIA., 2014,. 62(3): 467-482.
11
Mirjalili BF, Zamani L. Nano-TiCl4. SiO2: a versatile and efficient catalyst for synthesis of dihydropyrimidones via Biginelli condensation. S. Afr. J. Chem., 2014,. 67: 21-26.
12
Zamani L, Mirjalili BF, Namazian M. One-pot synthesis of 2, 4, 5-trisubstituted-1H-imidazoles promoted by nano-TiCl. Chemija., 2013,. 24(4): 312-319.
13
Zamani L, Mirjalili BF, Zomorodian K, Khabnadideh S. one pot preparations 1-amidoalkyl-2-naphthols derivative. JPSI., 2014,. 3(3): 208-216.
14
Mirjalili BF, Bamoniri A, Zamani L. One-pot synthesis of 1, 2, 4, 5-tetrasubstituted imidazoles promoted by nano-View the MathML source. Scientia Iranica C., 2012,. 19(3): 565-568.
15
Mirjalili BF, Bamoniri A, Zamani L. Nano-TiCl4/SiO2: An Efficient and Reusable Catalyst for the Synthesis of Tetrahydrobenzo [a] xanthenes-11-ones. Lett. Org. Chem., 2012,. 9(5): 338-343.
16
ORIGINAL_ARTICLE
Assessment of the Neural-Like Cells Differentiation from Endometrial Stem Cells following Fluoxetine Treatment
Fluoxetine is a common antidepressant which selectively inhibits serotonin reuptake at synaptic level. Some research findings have proposed the effect this drug on neurogenesis, neuronal survival as well as proliferation of the neural progenitor cells. Endometrium is a part of uterus which harbors mesenchymal stem cells. This source of stem cells can be differentiated into neural cells which may potentially be used in treating many diseases. Given the above, this study was designed to assess the effect of fluoxetine on neural cells differentiation from endometrial stem cells. Endometrial stem cells obtained from stem cell banking were cultured in Dulbecco’s modified eagle’s medium (DMED) containing fetal bovine serum (FBS) in the presence of Retinoic Acid, fluoxetine and Retinoic Acid+fluoxetine for 10 days. To assess the differentiation of endometrial stem cells into neural-like cells, we used immunocytochemistry and RT-PCR. The viability of cells was assessed using the trypan blue test. Data analysis revealed that 61% of endometrial stem cells differentiated to neural-like cells. Moreover, the biopotency of neural-like cells on fluoxetine treatment was more pronounced across differentiation days. Based on our findings, fluoxetine was shown to be a suitable inducer for the differentiation of neural-like cells from endometrial stem cells.
https://jamsat.sums.ac.ir/article_42454_2c6b268f11f326c0ec28c94f39b45d13.pdf
2015-12-31
105
111
10.18869/nrip.jamsat.1.2.105
Endometrial stem cell
Neural-like cells
Fluoxetine
Morteza
Motaghedifard
motaghedi.morteza@gmail.com
1
Kashan, Mofateh street, Firestation street,Postal address: 8714649783
LEAD_AUTHOR
Hamid Reza
Banafshe
banafshe57@hotmail.com
2
AUTHOR
Esmaeil
Fakharian
efakharian@gmail.com
3
AUTHOR
Javad
Verdi
jverdi2@yahoo.com
4
AUTHOR
Ali Reza
Shoa-Hassani
alirezashoae@gmail.com
5
AUTHOR
Krabbe C, Zimmer J, Meyer M, Neural trans differentiation of mesenchymal stem cells a critical review. APMIS 2005; 113:831-44.
1
Tysseling-Mattiace VM, Sahni V, Niece KL, Birch D, Czeisler C, Fehlings MG, Stupp SI, Kessler JA, Self-Assembling Nano fibers Inhibit Glial Scar Formation and Promote Axon Elongation after Spinal Cord Injury. The Journal of Neuroscience 2008; 28(14): 3814-23.
2
Meng XE, Ichim T, Zhong J, Rogers A, Yin Z, Jackson J, Wang H, Ge W, Bogin V, Chan KW, Thebaud B, Riordan NH, Endometrial regenerative cells: A novel stem cell population. Journal of Translational Medicine 2007; 5:57-63.
3
Gargett C, Masuda H: Adult stem cells in the endometrium. Molecular Human Reproduction 2012; 16 (11): 818-34.
4
Gargett CE, Uterine stem cells: What is the evidence? Health Iran TRIAL ACCESS on December 25, 2011.
5
Ai J, Tabatabaei FS, Jafarzadeh Kashi TS, Human Endometrial Adult Stem Cells May Differentiate Into Odontoblast Cells. Hypothesis 2009; 7(1): 21-9.
6
Hida N, Nishiyama N, Miyoshi Sh, Kira Sh, Segawa k,Uyama T, Mori T, Miyado K, Ikegami Y, Cui Ch, Kiyono T, Kyo S, Shimizu T, Okano T, Sakamoto M, Ogawa S, Umezawa A, Novel Cardiac Precursor-Like Cells
7
from Human Menstrual Blood-Derived Mesenchymal Cells. Stem cells 2008; 26:1695-04.
8
Patel A, Park E, Kuzman M, Benetti F, Silva F, Allickson J, Multi potent Menstrual Blood Stromal Stem Cells: Isolation, Characterization, and Differentiation. Cell Transplantation 2008; 17: 303-11.
9
Ai J, Tabatabaei FS, Kajbafzadeh AM, Myogenic Potential of Human Endometrial Adult Stem Cells. Iran J Med Hypotheses Ideas 2009; 3:25-32.
10
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26
ORIGINAL_ARTICLE
Demystifying the Biophoton-Induced Cellular Growth: A Simple Model
Background: None-Chemical Distant Cellular Interactions (NCDCI) are among the unexplained issues in cell biology. One example of such interactions is the biophoton-induced growth. In this process, photon emissions from one cell can induce mitosis in other cells while they are chemically separated. This effect is evident among many species. Hypothesis: It is hypothesized that some simple but universal molecular pathways, which include photoreceptor proteins, modulators of cell cycle and circadian rhythm, can explain this phenomenon. Particularly, existing experimental data has been used to support the hypothesis that exposure of cellular structures to visible light photons deactivates the cryptochrome protein and this deactivation disinhibits cell growth. This disinhibition happens through the influx of Ca2+ cations and subsequent activation of the downstream mitogenic pathways. Conclusion: While the existing lines of evidence are mixed and equivocal, current hypothesis provides a testable framework for further experimental investigation. The present model and its predictions can be used as a well-documented platform to address the mechanisms of None-Chemical Distant Cellular Interactions in biological systems.
https://jamsat.sums.ac.ir/article_42447_af0bf2d43ef00d421825e34ee167b316.pdf
2015-12-31
112
115
10.18869/nrip.jamsat.1.2.112
None-Chemical Distant Cellular Interactions (NCDCI)
Biophoton
Cryptochrome
Cell growth
Abolfazl
Alipour
alipour.abolfazl@yahoo.com
1
Conscioustronics Foundation, Shiraz, Iran
Student Research Committee, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
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38
ORIGINAL_ARTICLE
Immunotherapy to Address Unmet Needs in Oncology; Two Clinical Vignettes of Response in Metastatic Malignant Melanoma and Non-Small Cell Lung Cancer
The management of unresectable advanced, metastatic malignant melanoma (MM) and chemotherapy-resistant or refractory non-small cell lung cancer (NSCLC) has been an uphill challenge in clinical oncology. The advent of immunotherapy in cancer has put forward some new hopes to cover unmet needs in treating such cases. Immune-checkpoint inhibitors are among the well-supported options in the same vein. Alongside other cancer immunotherapy class-molecules, pembrolizumab (PZB), a programmed cell death protein 1 (PD-1) blocking antibody, has recently been approved both for MM and NSCLC. This report presents an overview of the clinical benefits of PZB in MM and NSCLC and highlights the clinical features of two eligible cases who have undergone immunotherapy using PZB.
https://jamsat.sums.ac.ir/article_42448_8236dc7ea2a3f6495e11377d0526d052.pdf
2015-12-31
116
119
10.18869/nrip.jamsat.1.2.116
Malignant melanoma
NSCLC
immunotherapy
Pembrolizumab
Clinical response
Arafat
Tfayli
at35@aub.edu.lb
1
NK Basile Cancer Institute, American University of Beirut Medical Center, Beirut, Lebanon
AUTHOR
Mohammad
Torabi-Nami
2
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences
LEAD_AUTHOR
Atabak
Ghotb
a_ghotb@behestandarou.com
3
Behphar Scientific Committee, Behphar Group, Tehran
AUTHOR
Hutchinson L. Immunotherapy: pembrolizumab-is the writing on the wall for cancer? Nature reviews Clinical oncology. 2015;12(7):371.
1
Guilleminault L, Carmier D, Heuze-Vourc'h N, Diot P, Pichon E. [Immunotherapy in non-small cell lung cancer: inhibition of PD1/PDL1 pathway]. Revue de pneumologie clinique. 2015;71(1):44-56.
2
Flemming A. Cancer: PD1 makes waves in anticancer immunotherapy. Nature reviews Drug discovery. 2012;11(8):601.
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Kline J, Gajewski TF. Clinical development of mAbs to block the PD1 pathway as an immunotherapy for cancer. Current opinion in investigational drugs. 2010;11(12):1354-9.
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Robert C, Schachter J, Long GV, Arance A, Grob JJ, Mortier L, et al. Pembrolizumab versus Ipilimumab in Advanced Melanoma. The New England journal of medicine. 2015;372(26):2521-32.
5
Pembrolizumab FDA approval. Available from: http://www.accessdata.fda.gov/drugsatfda_docs/label/2014/125514lbl.pdf.
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Garon EB, Rizvi NA, Hui R, Leighl N, Balmanoukian AS, Eder JP, et al. Pembrolizumab for the treatment of non-small-cell lung cancer. The New England journal of medicine. 2015;372(21):2018-28.
7
Mony JT, Zhang L, Ma T, Grabosch S, Tirodkar TS, Brozick J, et al. Anti-PD-L1 prolongs survival and triggers T cell but not humoral anti-tumor immune responses in a human MUC1-expressing preclinical ovarian cancer model. Cancer immunology, immunotherapy : CII. 2015;64(9):1095-108.
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21
ORIGINAL_ARTICLE
Genomic DNA Extraction from Sperm
Unlike the somatic cells, sperm DNA is very compact due to replacement of histones with protamines. Disulfide bridges formed within and between the protamines inhibit the extraction of sperm DNA through standard techniques used for the somatic cells. Furthermore, the spermatozoa themselves are protected by a membrane which is rich in disulfide bonds, making cell lysis very difficult. Following a comprehensive literature search, we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion and PCR amplification.
https://jamsat.sums.ac.ir/article_42449_fa86225d79042e17a2a0801dc96fb3b0.pdf
2015-12-31
120
121
10.18869/nrip.jamsat.1.2.120
Sperm
Disulfide bonds
Chromatin
Histones
Protamines
Zahra
Anvar
zahraanvar2000@yahoo.com
1
Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
Bahia
Namavar-Jahromi
2
Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Samaneh
Ebrahimi
ebrahimi8821@gmail.com
3
Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran
AUTHOR
Behrouz
Gharesi-Fard
gharesifb@sums.ac.ir
4
Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran
AUTHOR
Oliva R. Protamines and male infertility. Hum Reprod Update 2006; 12: 417-435
1
Cree LH, Balhorn R, and Brewer LR. Single Molecule Studies of DNA-Protamine Interactions. Protein Pept Lett 2011; 18: 802-810
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Griffin J. Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies. Methods Mol Biol 2013;927:379â384
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Wu H, De Gannes MK, Luchetti G, and Pilsner JR. Rapid method for the isolation of mammalian sperm DNA. Biotechniques 2015; 58:293-300
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8
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9
Marques C, Costa P, Vaz B, Carvalho F, Fernandes S, et al. Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia. Mol Hum Reprod 2007; 14: 67-74
10